WebHello, you can melt Chromotek GFP-TRAP at 95*С and use magnet to remove magnetic agarose beads as you described without problem. Cite 3 Recommendations 21st Jan, 2024 Adam Thiesen Massachusetts... WebSep 16, 2024 · ChromoTek GFP-Trap® magnetic agarose affinity beads ( see Note 2 ). 2. Low protein binding microcentrifuge tubes (e.g., LoBind® tubes). 3. Magnetic separation rack (should fit 1.5-ml microcentrifuge tubes). 4. Table-top centrifuge (cooled, with rotors suitable for 1.5- and 2-ml microcentrifuge tubes, RCF:19,000 × g ). 5.
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WebChromoTek Nano-Traps are the benchmark in immunoprecipitation. They guarantee fast, reliable and effective pull-downs of proteins and interacting partners, and have been cited in 4,000+... WebSep 7, 2024 · ChromoTek GmbH (Munich, Germany) offers nanobody based real time assays to visualize and measure the action of candidate drugs or other substances of interest on important regulatory processes in living cells. Presently, four Chromobody ® plasmids are available for analyzing the cell cycle, apoptosis, DNA methylation and … diabetic friendly mixed berry smoothie
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WebDec 24, 2015 · The precipitate was washed six times with extraction buffer and eluted in 2× Laemmli buffer. Anti-GFP Trap-A beads (gta-10; Chromotek) were used for IP. For protein immunoblots, proteins were separated by 12% SDS-PAGE and transferred to polyvinylidene fluoride membrane (Roth) by semidry blotting in 25 mM Tris-HCl, pH 8.3, 150 mM … WebThe high binding rate of the ChromoTek GFP-Trap enables a considerable shorter processing time than conventional anti-GFP-antibodies. After washing, the immunocprecipitated protein fraction may be subject to Western blotting, mass spectrometry analysis, ChiP, iCLIP, RIP or enzyme activity assays. WebGFP Nanobody Magnetic Agarose, ChromoTek GFP-Trap® from Proteintech Group Inc This product is no longer available on Biocompare. Product Specs Item GFP Nanobody … cindy tobin