Clonexpress multis one step cloning

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WebJul 25, 2024 · 232I and 232T pHAGE plasmids were previously constructed (Xu et al., 2016). mTFP was fused to N-terminal of either 232I or 232T in a pHAGE backbone by ClonExpress MultiS One Step Cloning Kit (Catalog#C113, Vazyme, China). Stable mTFP-232I/mTFP-232T expressing A20II1.6 B cell lines were acquired by lentivirus infection … WebMay 19, 2024 · ClonExpress ® II One Step Cloning Kit and ClonExpress ... These three fragments and the skeleton fragment are assembled via the isothermal assembly method using a ClonExpress ® MultiS One Step Cloning Kit (Vazyme, Nanjing, China). Table 2 KOD-plus-neo PCR system and temperature program for proofreading PCR amplification.

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WebOct 16, 2024 · To generate pCAMBIA1300-3xFLAG-NbAGO4-1, pCAMBIA1300 was digested with XbaI and XmaI, and then the 3xFLAG and NbAGO4-1 coding sequences were amplified by PCR and Infusion-cloned into pCAMBIA1300 with ClonExpress MultiS One Step Cloning Kit (Vazyme). pDONR207-Fibrillarin (Kim et al., 2007b) was used to … WebThe corresponding primers used for gene cloning are listed in Supplementary Table 1. The PCR products were purified and cloned into the EcoRI and ... digested pTAex3 using the ClonExpress® MultiS One Step Cloning Kit. For constructing plasmids harboring two or three genes, DNA fragments, including the amylase promoter and terminator, were ... league of legends wall banners

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WebClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Use any method to linearize … WebJul 9, 2024 · Clone a 3.0 kb SUR cassette from pCB1532 (fungal genetics stock center, FGN44:52-53) using the primers SUR-F and SUR-R. 3. Join the two flanking fragments, SUR cassette, and linearized pKO1B together to generate the knockout vector following the manufacturer’s protocols of ClonExpress MultiS One Step Cloning Kit. Web陈合凤，张帅，王喜军，王金良，温志远，葛金英，陈伟业*，步志高,2* （1.中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室，黑龙江哈尔滨 150069；2.江苏高校动物重要疫病与人兽共患病防控协同创新中心，江苏扬州 225009） league of legends wagers

ClonExpress II One Step Cloning Kit C112 - Vazyme Biotech

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WebClonExpress Ultra One Step Cloning Kit (Vazyme, #C115) ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113) Mut Express II Fast Mutagenesis Kit V2 (Vazyme, #C214) Mut Express MultiS Fast Mutagenesis Kit V2 (Vazyme, #C215) Find Similar Products by Category. Vazyme Molecular Research; WebIt can efficiently clone 50 bp - 10 kb fragments and carry out one step multi-fragment seamless cloning. Enzyme digestion is unnecessary. It only needs to introduce the … league of legends weakest championWebProduct Description. ClonExpress II One Step Cloning (Vazyme, #C112) is a simple, fast, and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of … league of legends warring kingdoms

"WebProduct Introduction. Mut Express® II Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, the target plasmid amplification product is digested with DpnI, ClonExpress® recombination and circularized, and then directly transformed to complete the site-directed mutation. " - Clonexpress multis one step cloning

Clonexpress multis one step cloning

WebCellagen Tech WebClonExpress II One Step Cloning Kit C112. ... Mut Express MultiS Fast Mutagenesis Kit V2 C215. USD$ 185.00. 5 min TOPO-Blunt Cloning Kit C602. USD$ 164.00. The products on this website may not be sold in all countries or regions. Please contact Vazyme or your local distributor for ...

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WebClonExpress MultiS One Step Cloning Kit 非连接酶依赖型的多片段一步克隆技术可在几乎任何载体的任意位点进行多片段拼接克隆无需考虑插入片段自身携带的酶切位点可一次实现二至五个插入片段的顺序拼接线性化克隆载体和 PCR 产物可不进行纯化直接 ... WebMar 10, 2024 · The ClonExpress MultiS One-step Cloning Kit was purchased from Vazyme (NanJing, China). All chemicals were reagent grade and obtained from Sigma–Aldrich (St. Louis, MO, USA). Table 1. Strains and plasmids used in this study. Strains and plasmids Description Source; Strains:

WebProduct Description. ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113) is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer in this kit are … WebJan 9, 2024 · The corresponding primers used for gene cloning are listed in Additional file 1: Table S1. The PCR products were purified and cloned into the EcoRI and KpnI linearized pTAex3 vectors using the ClonExpress ® II One Step Cloning Kit according to the manufacturer’s protocol to yield corresponding single gene expression plasmids.

WebMar 14, 2024 · Download PDF Copy. ClonExpress ® technology is a fast, simple and efficient DNA seamless cloning technology, enabling the insert of any position of any … WebClonExpress ® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Linearize the …

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WebClonExpress UItra One Step Cloning kit (Vazyme, #C115) is a novel cloning kit, applicable for homologous recombination with 1 - 5 fragments. The Kit is independent of DNA ligase, significantly reducing the self-ligated colonies. ... ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113) Mut Express II Fast Mutagenesis Kit V2 (Vazyme, … league of legends whats a good lp gain redditWebClonexpresstm Ii One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - … league of legends weaponWebThe ClonExpressTM MultiS (One Step Cloning) Kit provides a convenience, fast, high-throughput cloning of DNA fragment and generation of precise, directional constructs … league of legends warriors lyricsWebClonExpress II One Step Cloning Kit C112. USD$ 217.00. Ultra-Universal TOPO Cloning Kit C603. USD$ 196.00. Mut Express MultiS Fast Mutagenesis Kit V2 C215. USD$ 185.00. The products on this website may not be sold in all countries or ... league of legends what is itWebVazyme Biotech Co cloneexpress ii multis one step cloning kit. Cloneexpress Ii Multis One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz … league of legends welcome missionsWebIt can efficiently clone 50 bp - 10 kb fragments and carry out one step multi-fragment seamless cloning. Enzyme digestion is unnecessary. It only needs to introduce the homologous sequence at the end of the vector at the 5' end of the primer. The linearized clone vector and PCR products can be cloned directly without purification. league of legends wedding cakeWebJul 8, 2024 · Briefly, upstream and downstream sequences of target gene were amplified from mutants and purified PCR products were cloned into the pMo130-Hyg R vector using ClonExpress® MultiS One Step Cloning Kit (Vazyme biotech Co, Nanjing, China) via BamHI and XbaІ restriction sites. The primers used are listed in Supplementary Table 1. league of legends what is eternals