Cst c36b11
WebApr 9, 2024 · H3K27me3 [C36B11] $ 416.00 – $ 1,169.00. Format. Choose an option Concentrate ONCORE Pro Predilute Q Series UltraLine. Volume. Choose an option 0.1 …
Cst c36b11
Did you know?
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb, or Normal Rabbit IgG #2729, using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human GAPDH Exon 1 Primers #5516, SimpleChIP ® Human RPL30 Exon 3 Primers ... WebApr 12, 2024 · One microgram of acid-extracted protein was run on a 8 to 20% precast protein gel (Bio-Rad), transferred onto a nitrocellulose membrane, and probed with rabbit polyclonal anti-H3K27M (1:1000; ABE419, Millipore), rabbit monoclonal anti-H3K27me3 (1:1000; C36B11, CST), or rabbit polyclonal H3 (1:1000, ab1791, Abcam) antibodies. …
WebTarget Information. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. WebMonoclonal Antibody for studying H3 (Lys27) trimethylate. Cited in 14 publications. Validated for Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously …
WebH3K27me3 [C36B11] $ 416.00 – $ 1,169.00. Format. Choose an option Concentrate ONCORE Pro Predilute Q Series UltraLine. Volume. Choose an option 0.1 ml 1.0 ml 6.0 ml 60 Tests 7 ml. Clear. Add to cart. SKU: … WebFeb 1, 2024 · Mapping of protein binding sites in the genome is an essential tool in the fields of chromatin biology and gene regulation . Chromatin immunoprecipitation followed by high-throughput sequencing has been the most widely used method for many years, but various alternatives have been developed, each one with its own strengths and weaknesses …
WebNov 1, 2024 · To ensure the specificity of the H3K27me3 antibody (clone CST C36B11), we performed internal standard calibrated chromatin immunoprecipitation (ICeChIP; ref. 10), which demonstrated the high specificity of this antibody (Supplementary Fig. S1C). Figure 1. View large Download slide.
Webcombined with the bridging antibody Abcam ab6709 (see Note 14)) and H3K27me3 (CST C36B11, rabbit, 1: 100 dilution) in human HAP-1 cells. Additionally, the m6A distribution … fmea of pumpWebExample patterns are shown for Lamin B2 (Abcam ab8983, mouse, 1:100 dilution; combined with the bridging antibody Abcam ab6709 (see Note 14)) and H3K27me3 (CST C36B11, … fmea of sea transportationWebAug 28, 2024 · (B) ICeChIP-seq-based specificity measurement of αH3K27me3 (CST C36B11) antibody. Specificity is expressed as a fraction of normalized H3K27me3 … greensborough to kynetonWebchip antibody: H3K27me3 (clone C36B11, CST 9733) Extracted molecule: genomic DNA: Extraction protocol: Briefly, approximately 1×10^7 cells were fixed with 1% formaldehyde for 10 min and quenched by glysine. The cells were washed three times with PBS, then harvested in ChIP lysis buffer (50 mM Tris-HCl, pH7.6, 1 mM CaCl2, 0.2% Triton X-100) … fmea reverseWebSep 3, 2024 · This Histone H3 acetyl Lys27 antibody was raised against a peptide including acetyl-lysine 27 of histone H3. Uses : ChIP, ChIP-chip, ChIP-Seq, DB, IF, IP, WB. Storage : Antibodies in solution can be stored at -20M-BM-0C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. greensborough to lilydaleWebCell Signaling Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb 20 ul - CSIG (Additional S&H or Hazmat Fees May Apply) Cell Signaling Tri-Methyl-Histone H3 (Ly; CSIG-9733T The store will not work correctly in the case when cookies are disabled. fmea reworkWebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-Histone H3 (tri methyl K4) antibody [EPR20551-225] - ChIP Grade (ab213224) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. greensborough to melbourne cbd