NettetWe explored the specificity of AO staining while studying nuclear apoptosis in Tetrahymena. Using AO alone or together with the vital nuclear stain Hoechst 33342 (HO), we find that lysosomes are generally clustered around the degenerating nucleus and that such nuclei are stained an orange-red color, like lysosomes. Nettet14. aug. 2012 · Staurosporine-treated neurons undergo different outcomes when SYTO 13 and Hoechst are used in time-lapse imaging. (A) Neurons stained with Hoescht (blue; top panels) visually undergo chromatin condensation within 4 h and remained PI-negative.In contrast, neurons visualized with 500 or 5 nM SYTO 13 (green) became PI-positive …
SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy
NettetHoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear … NettetTech Tip: Staining Cells with Hoechst or DAPI Nuclear Stains - Biotium Antibody $0 Tech Tip: Staining Cells with Hoechst or DAPI Nuclear Stains Want a printable copy of this protocol? Sign up for our e-newsletter to receive a link. Tech Tip: Staining Cells with Hoechst or DAPI Nuclear Stains First name * Last name * Company / Institution * Email * feelings ty amy
BioTracker 488 Green Nuclear Dye Live Cell Imaging Millipore
NettetOver the years a number of nuclear staining techniques such as propidium iodide, Hoechst-33342, 4', 6-diamidino-2-phenylindole (DAPI), Acridine orange-Ethidium bromide staining, among others have been developed to assess the changes in DNA. Nettet14. des. 2024 · Differentiated SH-SY5Y cells were labelled with βIII-tubulin and a Hoechst nuclear counterstain. βIII-tubulin images were acquired using the GFP filter (excitation 482/25 nm; emission 524/24 nm ... Nettetpermeable nuclear staining compound (i.e. Hoechst 33342), 2) automated imaging and cell counting optimized for XF assays, and 3) synchronization of images and cell counts into XF result files in Wave software. This solution provides an efficient and reliable method of normalization with a seamless and rapid workflow for XF Analyzer users. define inflation and how it is measured